Image from the 0 degree tilt angle of image set "A" from the double tilt data series.
Full resolution image description
Files that are needed / used for a TxBR reconstruction. Included with the files are the .preali file, the .rawtlt file, .fid file and all processing files.
Animation description
Tilt series of both the "A" and "B" stacks. The animation first shows the tilt series of A and then switches to the tilt series of stack B.
Produce a series of tomographic data of the normal morphology of Caulobacter Crescentus using rapid freezing methods.
Experimenter(s)
Patrick Viollier
Tom Deerinck
Microscopy product
Microscopy product ID
3628
Instrument
JEOL 4000
Microscopy type
IVEM
Product type
DOUBLE TILT
Image basename
caulo_double1
Spatial Axis
Image Size
Pixel Size
X
2242px
1 nm/pixels
Y
3340px
1 nm/pixels
Subject
Species
Caulobacter
Scientific name
Caulobacter crescentus
Strain
3724
Treatment
None
Age class
not applicable
Specimen description
Cell type
Caulobacter crescentus
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
30000
Accelerating voltage
400 kV
Specimen preparation
Protocol used
High Pressure Freezing (HPF): completed on January 26th, 2006 using Balzers HPM 010 in 100 micron deep gold plated copper planchettes. Bacteria was centrifuged decanted and paced applied to planchettes.Free Substitution: Started Friday January 26th.1. Cocktail - 2% Osmium tetroxide, 0.5% urynal acetate in anhydrous acetone2. Time -T1: -90 Celsius for 72 hoursS1: 2 Celsius per hour for 15 hoursT2: -60 Celsius per hour for 12 hoursS2: 5 Celsius per hour for 12 hoursT3: 0 Celsius HoldLeica FSU
Imaging product type
Type
Double tilt
X min range
-66 degrees
X max range
66 degrees
X tilt increment
2 degrees
Y min range
-66 degrees
Y max range
64 degrees
Y tilt increment
2 degrees
Description
Caulobacter Crescentus, 0.5 um thick, 400kv
Citation Information
Lucy Shapiro, Harley McAdams, Patrick H. Viollier, Tom Deerinck, Mason Mackey, John Crum, Mark Ellisman (2003) CCDB:3628, Caulobacter crescentus, Caulobacter crescentus. CIL. Dataset. https://doi.org/doi:10.7295/W9CCDB3628