A maximum intensity projection of a Caulobacter crescentus specimen at 0 degree tilt.
Full resolution image description
This .tar file contains two .mrc reconstructed volumes: cb1_full.rec and
cb1.rec. cb1_full.rec is the full, untrimmed (and unflipped) volume.
cb1.rec is the trimmed slice by slice volume used for subsequent analysis.
Volume_dimension
993, 1582, 101
Volume scale
0.001, 0.001, 0.001
Animation description
a .mpg animation movie of a maximum intensity projection of Caulbacter crescentus, rotating about the y-axis.
A Caulobacter crescentus specimen from the aligned tilt series at 0 degree tilt.
Full resolution image description
A .tar file containing all files necessary for a slice by slice volume
reconstruction using IMOD. Some files included are the .st, .preali,
.ali, .fid, .rawtlt and a notes.txt file listing some procedures used
during the reconstruction process.
Animation description
a .mpg file format movie of a Caulobacter crescentus. Movie involves the aligned tilt series.
To produce a series of tomographic reconstructions of the normal morphology of caulobacter using traditional fixing methods.
Experimenter(s)
Tom Deerinck
Guido Gaietta
Microscopy product
Microscopy product ID
3700
Instrument
JEOL 4000 #1
Microscopy type
IVEM
Product type
SINGLE TILT
Image basename
cb1
Spatial Axis
Image Size
Pixel Size
X
4020px
0.556 nm/pixels
Y
4020px
0.556 nm/pixels
Subject
Species
Caulobacter crescentus
Scientific name
Caulobacter crescentus (Snc04)
Strain
Snc04
Age class
N/A
Tissue section
Anatomical location
N/A
Tissue product storage
N/A
Thickness
0.4 µm
Specimen description
Cell type
Caulobacter crescentus
Imaging parameters
Type
Electron microscopy product
Recording medium
CCD
Magification
40000
Specimen preparation
Protocol used
Induced for 1 hour at 30 degrees CLabeled in M2G medium for 2 hours at 30 degrees C (in the presence of 1.8 micromolar ReAsH-EDT2) and photoconvertedSamples were fixed with 2.5% glutaraldehyde with 1% acrolein in 0.05M sodium cacodylate pH 7.4 with 3 mM CaCl2 and 1 % sucrose followed by conventional osmium tetroxide/potassium ferrocyanide post-fixation, dehydration and embedding.
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-60 degrees
Max range
60 degrees
Tilt increment
2 degrees
Citation Information
Lucy Shapiro, Harley McAdams, Patrick H. Viollier, Tom Deerinck, Mason Mackey, John Crum, Mark Ellisman (2003) CCDB:3700, Caulobacter crescentus (Snc04), Caulobacter crescentus. CIL. Dataset. https://doi.org/doi:10.7295/W9CCDB3700