Large scale mosaic of rat thalamus reconstructed through automated acquisition and stitching of over 457 tiles, triple labeled for GFAP (Red), Pannexin 1(Green) and DAPI (Blue), and imaged using laser scanning confocal microscopy. The contrast and brightness was adjusted for display purposes.
Full resolution image description
Large scale mosaic of rat thalamus reconstructed through automated acquisition and stitching of over 457 tiles, triple labeled for GFAP (Red), Pannexin 1(Green) and DAPI (Blue), and imaged using laser scanning confocal microscopy. The contrast and brightness was adjusted for display purposes.
A neuroinformatics approach to pannexin expression, localization, and interaction with other brain proteins. This research aims to examine brain tissue at high resolution and, in combination with developing neuronformatics tools, to probe for binding partners and signalling partners to investigate the pannexin interactome . Data submissions for this project will consist of wide-scale high resolution confocal mosaic images of triple-labeled whole mouse brain sections. Pannexin 1 expression will be compared to that of pannexin 2 as well as the connexins. Other putative interaction partners of interest include the P2 purinergic receptors and components of the cytoskeleton.