Single aligned electron micrograph of a 0.25 um thick section from a serial section reconstruction of the cell body through a cortical pyramidal neuron from the brain of a patient with Alzheimer's disease.
Full resolution image description
Aligned serial section reconstruction in IMOD MRC format. Image stack that was aligned using Midas from the IMOD package to correct for non-linear distortions. The image stack was then binned down by 4x for both the X and Y axis.
Electron micrograph of the cell body of a 0.25 um thick section through a cortical pyramidal neuron from the brain of a patient with Alzheimer's disease. The specimen was prepared from biopsy material obtained several decades ago.
Full resolution image description
Stack of Alzheimer neuronal images modified by IMOD using the newstack program.
Reconstruction of cortical neurons from biopsy material obtained from Alzheimers disease patients
Funding agency
National Institutes of Health
Leader(s)
Mark Ellisman
Collaborator(s)
Rama Ranganathan
Thomas J. Deerinck
Stephen J. Young
David Hessler
and Robert D. Terry
Start date
unspecified
End date
unspecified
Experiment
Experiment ID
3448
Experiment date
01-01-1987
Title
Alzheimer reconstruction
Purpose
Reconstruction of Alzheimers neurons using serial section EM
Experimenter(s)
Tom Deerinck
Microscopy product
Microscopy product ID
6289
Instrument
JEOL100CX
Microscopy type
TEM
Product type
SERIAL SECTION
Image basename
ALZ301_4
Subject
Species
human
Scientific name
homo sapiens
Strain
sapiens
Group by
disease status
Treatment
patients diagnosed with Alzheimers disease
Age class
aged
Tissue section
Anatomical location
cerebral cortex
Microtome
ultramicrotome
Thickness
0.25 µm
Specimen description
Organ
brain
System
central nervous system
Cell type
Neocortex pyramidal cell
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
3300
Accelerating voltage
100 KeV
Imaging product type
Type
Serial section
Z resolution
0.25 µm
Citation Information
Mark Ellisman, Rama Ranganathan, Thomas J. Deerinck, Stephen J. Young, David Hessler, and Robert D. Terry CCDB:6289, homo sapiens, Neocortex pyramidal cell. CIL. Dataset. https://doi.org/doi:10.7295/W9CCDB6289